Difference between revisions of "Davidson Missouri W/Davidson Protocols"
From GcatWiki
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# [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | # [http://www.promega.com/biomath/calc11.htm Promega T<sub>m</sub> Calculator] | ||
# [http://partsregistry.org/AHL List of auto-inducers and their catalog numbers] | # [http://partsregistry.org/AHL List of auto-inducers and their catalog numbers] | ||
| − | #[ | + | #[[Sequencing at Agencourt| Sequencing at Agencourt Bioscience]] |
#[[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | #[[Davidson Missouri W/CUGI_Seuqencing| Sequencing at CUGI]] | ||
#[http://gcat.davidson.edu/GcatWiki/images/3/3b/Ape_protocol.pdf Analyzing Sequences with aPe] | #[http://gcat.davidson.edu/GcatWiki/images/3/3b/Ape_protocol.pdf Analyzing Sequences with aPe] | ||
#[http://gcat.davidson.edu/IGEM06/oligo.html Lance-olator Oligos for dsDNA assembly] | #[http://gcat.davidson.edu/IGEM06/oligo.html Lance-olator Oligos for dsDNA assembly] | ||
#[http://gcat.davidson.edu/GCATalog-r2.3/StartPage.php Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks] | #[http://gcat.davidson.edu/GCATalog-r2.3/StartPage.php Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks] | ||
Revision as of 20:57, 16 July 2009
- How to Keep a Lab Notebook
- Common molecular reagents
- Standard Assembly
- BioBrick Ends
- Compatibility of Plasmids
- Building dsDNA with Oligos
- Setting up PCR mixtures
- PCR and Mg2+ concentration
- Clean and Concentrate DNA (after PCR, before digestion)
- Pouring an agarose gel
- Calculate MWs
- Digest DNA with restriction enzymes
- Double Digest Guide
- Ethanol Precipitate DNA (short protocol)
- 1kb MW markers
- Shrimp Alkaline Phosphatase
- Qiagen QIAquick Gel Purification
- Qiagen QIAquick Column Regeneration Protocol
- ElectroElute Gel Purification
- Ligation Protocol
- Heat Shock Transformation OR Short version of Heat Shock
- Zippy Transformation
- TSS Competent Cell Transformation
- Colony PCR to Screen for Successful Ligations
- Promega miniprep
- Choices for Transformation: Heat Shock vs. Zyppy
- Choices for Mini-Preps: Promega vs. Zyppy
- Making dsDNA Using Primer Dimers
- Genomic Insertion Protocol
- glycerolstocks How to Make Glycerol Stocks of Bacteria
- When inducing with IPTG, use 3 µL of stock (0.2 g/mL = 20% w/v) to every 1 mL of LB or other liquid.
- When inducing with Arabinose, use "2 µL of stock" (10% w/v L-Arabinose) "to every 1 mL" of LB or other liquid.
Computer Tools We Use
- All Sites In One Place
- Optimize your Gel
- Gene Splitting Web Site
- PCR Primers w/ BioBricks
- Promega Tm Calculator
- List of auto-inducers and their catalog numbers
- Sequencing at Agencourt Bioscience
- Sequencing at CUGI
- Analyzing Sequences with aPe
- Lance-olator Oligos for dsDNA assembly
- Access the GCAT-alog of Davidson and MWSU DNA Freezer Stocks
