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• Halorhabdus utahensis Genome
  ...chaea domain, that lives in environments containing high concentrations of salt [http://en.wikipedia.org/wiki/Halophile] (Matt)
  41 KB (6,169 words) - 19:29, 11 December 2008
• Will Notebook1
  ...e I run a gel I will replace the buffer, which seems to have an unbalanced salt concentration. Since most of the pLux' and pLuxLas colonies gave bands that
  40 KB (6,782 words) - 19:27, 19 December 2008
• Protein Export
  ...halophiles have a lot of K+ in cytoplasm to counter balance extracellular salt. Their proteins have many negative amino acids that help keep the proteins
  5 KB (595 words) - 16:15, 18 November 2008
• IGEM 2009 notebook
  ...restriction enzymes we were using and how effective they'd be in different salt solutions. Online on the Promega "Compatible Buffers" website, we were able 3. Clean and concentrate plasmids so salt solution is optimal for digestion.
  54 KB (9,611 words) - 20:15, 29 June 2009
• Halomicrobium mukohataei Genome Fall 2009
  How, exactly, do halophiles manage to live in such high-salt conditions?<br> [[2) What gene products]] allow halophiles to survive in high salt content?
  70 KB (10,394 words) - 13:19, 20 June 2011
• 7) Two 'other' RNA genes
  ...s that RNA modifications are very low in archaea possibly because the high salt concentration within the cell prevents mutation of nucleosides.
  833 bytes (133 words) - 06:35, 10 September 2009
• 2) What gene products
  ...a are so adapted to high-salt environments that they cannot survive in low-salt conditions. There are 6 different haloarchaeal genomes that have been seque ...gi/reprint/152/3/585 (Soppa 2006)] Halophilic proteins are adapted to high-salt by having more acidic residues than basic residues, or a high concentration
  2 KB (317 words) - 07:19, 10 September 2009
• Karen's Assignment
  ...to water, so the only way to prevent the loss of cellular water under high salt conditions is to increase the internal solute concentration. Halotolerantan ...um homeostasis and how it may be useful to our organism to survive in high salt concentrations by first researching how other known species utilize this me
  19 KB (2,788 words) - 13:21, 1 October 2009
• Claudia's Assignment
  ...daptations. There are a number of halophile species as well as a number of salt-tolerant organisms, including a number of bacteria and a few yeasts.<br> ...o compare these genes and the pumps they encode for to the genome of other salt-loving microorganisms, specifically ''Debaryomyces hansenii'', which has be
  12 KB (1,772 words) - 15:28, 1 October 2009
• Katie's Assignment
  ...genetic make up of enzymes that have been previously identified as being "salt dependent" for activity- such as citrate synthase, malic enzyme and asparta ...orrelated sequence within our species' genome is not from one of the other salt dependent enzyme genes.
  11 KB (1,436 words) - 23:37, 6 October 2009
• Sarah's Assignment
  ...gnificant similarity with our organism's DDP were also halophiles found in salt flats around the world. This suggests that maybe the changes which make our
  10 KB (1,567 words) - 15:58, 22 October 2009
• Degradation of Xenobiotics by Halomicrobium mukohataei (Megan Reilly)
  ...solved in aqueous solutions - polluting aquatic ecosystems. In salt lakes, salt marshes, or other areas with high salinity, halophiles may be an effective
  5 KB (868 words) - 15:32, 1 October 2009
• Comparison of Each Enzyme's Protein Sequences using blastp and clustalW. . .
  ...prove to show that these enzymes in our species are also dependent on high salt concentrations.
  777 bytes (100 words) - 02:20, 1 December 2009
• Citrate Synthase
  ...onas pharaonis'' - halophillic archaean - got rid of because is not just a salt-loving organism but can also survive high salinity '''The following are salt-loving bacertia with top hits'''
  13 KB (1,173 words) - 23:58, 6 October 2009
• Aspartate Carbamoyltransferase
  ''Natrialba magadii''- got rid of because i wanted to narrow search to just salt-loving and not other extremophiles '''Non-salt loving organisms with top hits'''
  9 KB (312 words) - 01:12, 7 October 2009
• Halorhabdus utahensis
  ...n more about it [http://www.destination360.com/north-america/us/utah/great-salt-lake click here]. This species grows best at 27% NaCl. Only a few sugars su
  1 KB (198 words) - 16:38, 20 October 2009
• Haloarcula sp 33799
  ...0 contigs in its partially sequenced genome. The species was discovered in salt brines in Baja California and Mexico. The genome at this point for this spe
  624 bytes (96 words) - 16:28, 15 October 2009
• Haloarcula vallismortis
  ...ml Basic Information on Sequencing and Environmental Conditions]- lives in salt pools, found collected from Bad Water Point, Death Valley, CA ...2/05/badwater-basin-death-valley-national-park/ Picture of Bad Water Point salt pools], where this organism is found:
  499 bytes (65 words) - 16:46, 15 October 2009
• Haloferax volcanii
  ...nyon.edu/index.php/Haloferax]. As with most halophiles, it requires a high salt concentration in order to thrive.
  2 KB (263 words) - 16:18, 20 October 2009
• Haloferax sulfurifontis
  ...content as compared to other Halobacteria, although it is best suited to a salt content of 25% or above [http://ijs.sgmjournals.org/cgi/content/full/54/6/2
  2 KB (251 words) - 16:34, 20 October 2009
• Haloferax denitrificans
  ...x'' are extremely halophilic, most are also pleomorphic and do not require salt levels as high as other halophiles for growth. ''Haloferax denitrificans''
  686 bytes (98 words) - 16:45, 27 October 2009
• Haloarcula sinaiiensis 33800
  ...iensis'' is a halophilic, antialkaliphilic organism that was isolated from salt brine in Israel.
  157 bytes (20 words) - 16:46, 29 October 2009
• Bacterial Media
  Weigh out 2.5 g Ampicillin, sodium salt (Sigma A0166)
  2 KB (248 words) - 18:49, 3 July 2014
• Blueberry Genome Project for Bio343
  .... Many Archaea are extremophiles, such as Halobacteria that thrive in high-salt environments (Lecture, Olivia) ...chaea domain, that lives in environments containing high concentrations of salt [http://en.wikipedia.org/wiki/Halophile] (Matt)
  129 KB (19,401 words) - 03:44, 19 May 2014
• Catherinel Notebook1
  The curvature of the band indicates a difference is salt concentration between the TBE in the box and in the gel. Also, the control
  147 KB (21,983 words) - 13:42, 24 January 2014
• Electroporation Transformation
  4. Click the Pulse button. If there is too much salt, the cuvette can arch and make a pop sound. [https://vimeo.com/user37250198
  2 KB (294 words) - 13:46, 21 January 2018
• Electroporation Old School Campbell
  # Inoculate a 50 mL flask of LB (low salt) with JM109 stock and grow overnight at 37˚C. ...5 mL of overnight JM109 into a 1 liter flask containing 500 mL of LB (low salt) and incubate at 37˚C and 400 rpm.
  2 KB (379 words) - 21:55, 23 June 2016
• Electroporation - Campbell Old School Method
  # Inoculate a 50 mL flask of LB (low salt) with JM109 stock and grow overnight at 37˚C. ...5 mL of overnight JM109 into a 1 liter flask containing 500 mL of LB (low salt) and incubate at 37˚C and 400 rpm.
  2 KB (386 words) - 22:01, 27 June 2016