Difference between revisions of "Build XOR Gate"
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[[Mnt/Tet]] | [[Mnt/Tet]] | ||
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+ | '''Combined Parts''' | ||
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+ | pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
+ | |||
+ | |||
+ | pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT | ||
+ | |||
+ | |||
+ | pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT | ||
+ | |||
+ | |||
+ | pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT | ||
+ | |||
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+ | These parts will be placed in a medium copy amp plasmid (probably pSB4A3) | ||
+ | |||
'''Overlapping Parts And intermediates''' | '''Overlapping Parts And intermediates''' |
Latest revision as of 15:24, 6 August 2008
Combined Parts
pLux/cI+RBS+LuxS+RBS+Mnt+TT+pLac/Mnt+RBS+LuxS+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLsr/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pBAD+RBS+LsrK+TT
pLux/cI+RBS+LasI+RBS+Mnt+TT+pLac/Mnt+RBS+LasI+RBS+cI+TT+pBAD+RBS+LacI+TT+pBAD+RBS+LuxR+TT
pLas/cI+RBS+LuxI+RBS+Mnt+TT+pTet/Mnt+RBS+LuxI+RBS+cI+TT+pBAD+RBS+TetR+TT+pLacI+RBS+LsrR+TT
These parts will be placed in a medium copy amp plasmid (probably pSB4A3)
Overlapping Parts And intermediates
LuxR/cI | Lac/Mnt | LsrR/cI | LasR/cI | Mnt/Tet |
---|---|---|---|---|
C0261(RBS+LuxI) | C0261(RBS+LuxI) | C0261 (RBS+LuxI) | ||
S0103(RBS+cI) | S0103(RBS+cI) | |||
I13025(RBS+mnt) | I13025(RBS+Mnt) | I13025(RBS+Mnt) | ||
K091109(LuxS) | K091109(LuxS) | |||
RBS+Mnt+TT | RBS+Mnt+TT | RBS+Mnt+TT | ||
RBS+cI+TT | RBS+cI+TT | |||
RBS+luxS | RBS+luxS | |||
RBS+lasI | RBS+lasI | |||